cihr_grants: 170530

This data as json

external_id	title	project_lead_name	co_researchers	institution	province	country	competition_year	award_amount	program	program_type	theme	research_subject	keywords	abstract	duration	source_url
170530	Targetting and Regulation of Ion Transporters	Grinstein Sergio	Grinstein, Sergio	Hospital for Sick Children (Toronto)	Ontario	Canada	200809	939768.0	Operating Grant	Operating Grants	Biomedical	Circulatory and Respiratory Health	Acid-Base Regulation; Fluorescence Imaging; Ion Transport; Na/H Exchange; Ph Regulation	The acid-base balance of organisms, including humans, must be carefully regulated. Both the pH (a measure of the concentration of acid) of cells and of the fluids that bathe them, including blood, are continuously challenged, yet maintained within very narrow boundaries. This exquisite regulation is attributable to a family of molecules that transport acid or base across the cell membranes. Among the most active and important transporters are the sodium/proton exchangers, a family of 9 different types of molecules widely distributed throughout the body. Several of these have been linked to various types of neurological, vascular and renal disease. The work described in this proposal has three separate aims: 1) Understanding how one of the prototypical sodium/proton exchangers, NHE3, interacts with and is regulated by the charge of the cell membrane; 2) To use yeast as a vehicle to express and study the NHE6-9 exchangers, which have proven otherwise intractable and 3) To devise and implement a method to study the electrical potential that develops across the membrane of endosomes, organelles that carry cargo into the cells. This fundamental knowledge will contribute to our understanding and eventual treatment of disease.	5 yrs 0 mth	https://webapps.cihr-irsc.gc.ca/decisions/p/project_details.html?applId=170530&lang=en